The purpose of molecular pathology is to elucidate the mechanisms of disease by identifying molecular and pathway alterations. At the core of this still-evolving discipline is the application of classical and novel techniques developed in biochemistry, cell biology, molecular biology, proteomics, and genetics, to the evaluation of pathological processes. Within the pharmaceutical industry, the effectiveness of molecular pathology as an adjunct to histopathology in nonclinical drug development is well recognized. Many of the techniques of molecular pathology rely on the use of labeled antibodies and nucleic acid probes and are either slide-or fluid-based. In this chapter, we provide brief descriptions of the basic principles underlying the slide-based techniques and emphasize their applications in the nonclinical phase of drug development.
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